Request Your Free CleanNGS Sample – The All-in-One NGS Library Cleanup and Size Selection Solution

A new standard for DNA & RNA library purification

Are you performing Next-Generation Sequencing? In order to achieve optimal results, library purification and size selection are an essential step of sample preparation. Whether your NGS workflow is for DNA or RNA sequencing, in diagnostics or for research purposes: by choosing our CleanNGS magnetic beads for NGS, you can’t go wrong.

DNA and RNA

Fast and efficient

Easy automation

Ambient storage

Suitable for NGS

Available for diagnostics procedures (CE-IVD) or research

Request your free sample here!

Removal of impurities and selection of the fragment sizes of choice are critical steps in a library preparation process. Without proper cleanup, impurities can inhibit key enzymes used in library prep or clog flow cells in sequencing reactions. Very short or long fragments can lead to poor-quality reads, adapter contamination or low yield.

Our CleanNGS reagent is an affordable one-on-one replacement for Beckman AMPure XP, SPRI Select and RNA Clean XP. It is basically 3 products in one for your NGS sample prep!

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Proof of Principle

Two different double-size selections (1,00/0,70 and 0,85/0,56) were performed on sheared human genomic DNA. We compared CleanNGS, AMPure XP and SPRI Select, see Figure 1. The results show that CleanNGS performs identical to AMPure XP as well as the SPRI beads. CleanNGS provides consistent double-size selection results and efficiently excludes fragments above and below the target cutoff regions.

Figure 1.

Average DNA fragment size after double-size selection.

In another experiment, CleanNGS, AMPure XP and Illumina SPB beads were used for purification and double-size selection in a Nextera DNA Flex Library Prep. The generated DNA libraries were sequenced using an Illumina MiSeq and the quality scores of the Read 1 and Read 2 traces were determined. CleanNGS demonstrated equal performance versus AMPure XP and Illumina SPB.

Figure 2.

Average Sequencing Quality score determined of both Read 1 and Read 2 on an Illumina MiSeq instrument.

Amazing results!

10/03/2024

These beads are easy to use, and are extremely effective in our applications. The beads produce high quality results and also produce extremely reproducible work. My research would not be the same without these cost-effective and efficient beads. I also appreciate how friendly and the willingness to help that all of the sales team has at GC Biotech to help provide me with what I want.

Michelle Michelsen

Very happy and will keep using them.

29/06/2020

Some members of the lab were not keen to change away from the regular bead clean up supplier. We tested these and found they gave slightly higher amount of DNA and just as clean.

Chris Kimberley

Like-for-like replacement to the large name-brand equivalents.

16/01/2020

Delivers reproducible DNA size-selection for a variety of NGS applications and can be used in with-bead applications.

Patrick van Eijk

What does a sample preparation workflow with CleanNGS look like?

CleanNGS is there for you, every step of the way.

Step 1 - Fragmentation
After DNA or RNA extraction, the nucleic acids are sheared mechanically or with enzymes to achieve the required fragment sizes for NGS. RNA is converted to cDNA during or after this step. Fragment sizes can vary per sequencing instrument used, for example from 200-500 bp for Illumina and up to 1 MB for Oxford Nanopore.

To remove reaction buffers or unwanted small fragments, an optional cleanup with CleanNGS can be performed.

Step 2 - End-repair & Adapter ligation
The DNA fragments are converted into blunt-ended, A-tailed DNA/RNA for adapter ligation.

Enzymes, salts, and unwanted reaction byproducts are removed in a CleanNGS cleanup step.

Step 3 - Double size selection
Now is the time for CleanNGS to perform a double-size selection of the DNA fragments. Our reagent provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).
Step 4 - PCR amplification
If needed, a PCR amplification step enriches the library by using platform-specific primers.

A final CleanNGS purification step removes PCR byproducts, such a primer-dimers.

Step 5 - Sequencing
The clean libraries are loaded onto Illumina, Oxford Nanopore or another next-generation sequencer of choice.

Request your free sample here!

Order info
Product Preps Part number
CleanNGS 1 mL 55 CNGS-0001
CleanNGS 50 mL 2,777 CNGS-0050
CleanNGS 500 mL 27,777 CNGS-0500
Product Preps Part number
CleanNGS Dx 50 mL 2,777 CNGSDx-0050
CleanNGS Dx 500 mL 27,777 CNGSDx-0500

Thank you!

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