Request Your Free CleanNGS Sample

A magnetic bead-based solution for DNA & RNA purification – available as CE-IVD or RUO, and a smart alternative to AMPure XP and SPRI beads.

NGS Cleanup & Size Selection Made Easy

CleanNGS is your all-in-one reagent for fast, cost-effective purification and double-sided size selection of DNA and RNA.

Replaces AMPure XP, SPRI Select, and RNA Clean XP – all in one bottle.

Compatible with Illumina, Nanopore & automation
No centrifugation, no columns – just magnetic beads
Excellent yield, high reproducibility
Ambient storage – no cold chain needed
Available for research and CE-IVD clinical workflows

“CleanNGS gave higher DNA yields and consistent results.” – Chris K.

“Cost-effective and easy to automate.” – Michelle M.

Request your free sample here!

Proven Performance: CleanNGS Matches The Golden Standard

Two different double-size selections (1,00/0,70 and 0,85/0,56) were performed on sheared human genomic DNA. We compared CleanNGS, AMPure XP and SPRI Select, see Figure 1. The results show that CleanNGS performs identical to AMPure XP as well as the SPRI beads. CleanNGS provides consistent double-size selection results and efficiently excludes fragments above and below the target cutoff regions.

Figure 1.

Average DNA fragment size after double-size selection.

In another experiment, CleanNGS, AMPure XP and Illumina SPB beads were used for purification and double-size selection in a Nextera DNA Flex Library Prep. The generated DNA libraries were sequenced using an Illumina MiSeq and the quality scores of the Read 1 and Read 2 traces were determined. CleanNGS demonstrated equal performance versus AMPure XP and Illumina SPB.

Figure 2.

Average Sequencing Quality score determined of both Read 1 and Read 2 on an Illumina MiSeq instrument.

Amazing results!

These beads are easy to use, and are extremely effective in our applications. The beads produce high quality results and also produce extremely reproducible work. My research would not be the same without these cost-effective and efficient beads. I also appreciate how friendly and the willingness to help that all of the sales team has at GC Biotech to help provide me with what I want.

Michelle Michelsen

Good quality and value for money

We have been using cleanNGS for several years now at ILVO for clean-up of Illumina targeted sequencing libraries and clean-up of Nanopore WGS libraries. It replaces the AMPureXP beads 1:1 for several workflows and we are very happy with the consistently high quality, good value for money and simple protocol.

Steve Baeyen

Very happy and will keep using them.

Some members of the lab were not keen to change away from the regular bead clean up supplier. We tested these and found they gave slightly higher amount of DNA and just as clean.

Chris Kimberley

What does a sample preparation workflow with CleanNGS look like?

CleanNGS is there for you, every step of the way.

Step 1 - Fragmentation

After DNA or RNA extraction, the nucleic acids are sheared mechanically or with enzymes to achieve the required fragment sizes for NGS. RNA is converted to cDNA during or after this step. Fragment sizes can vary per sequencing instrument used, for example from 200-500 bp for Illumina and up to 1 MB for Oxford Nanopore.

To remove reaction buffers or unwanted small fragments, an optional cleanup with CleanNGS can be performed.

Step 2 - End-repair & Adapter ligation

The DNA fragments are converted into blunt-ended, A-tailed DNA/RNA for adapter ligation.

Enzymes, salts, and unwanted reaction byproducts are removed in a CleanNGS cleanup step.

Step 3 - Double size selection

Now is the time for CleanNGS to perform a double-size selection of the DNA fragments. Our reagent provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).

Step 4 - PCR amplification

If needed, a PCR amplification step enriches the library by using platform-specific primers.

A final CleanNGS purification step removes PCR byproducts, such a primer-dimers.

Step 5 - Sequencing

The clean libraries are loaded onto Illumina, Oxford Nanopore or another next-generation sequencer of choice.

Request your free sample here!

Order info
Product	Preps	Part number
CleanNGS 1 mL	55	CNGS-0001
CleanNGS 50 mL	2,777	CNGS-0050
CleanNGS 500 mL	27,777	CNGS-0500

Product	Preps	Part number
CleanNGS Dx 50 mL	2,777	CNGSDx-0050
CleanNGS Dx 500 mL	27,777	CNGSDx-0500