CleanNGS

CleanNGS
DNA and RNA clean-up for next generation sequencing library construction

The CleanNGS kit offers a highly efficient magnetic bead based clean-up system for the purification of both DNA and/or RNA for next-generation sequencing workflows. CleanNGS provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).
Based on our proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications such. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.

Features:

  • Designed for both DNA and RNA purification
  • Ideal for (double-sided) size selection for Next-Generation Sequencing
  • Efficient removal of unincorporated dNTPs, primers, primer dimers and other contaminants
  • No centrifugation or filtration
Amplicons purified with CleanNGS system are ready to be used in the following applications:

  • Next-Generation Sequencing
  • PCR
  • Genomic DNA clean up
  • RNA clean up
  • Fragment Analysis
  • Microarrays
  • Restriction enzyme clean up
  • Cloning

Available kit formats

Catalog No#

Description

Number of Reactions*

CNGS-0005

CleanNGS (5 mL)

277

CNGS-0050

CleanNGS (50 mL)

2,777

CNGS-0500

CleanNGS (500 mL)

27,777

*Based upon a 10 ┬Ál PCR reaction volume