Is your lysis buffer precipitated? Heat it at 37 °C before starting your experiment. To prevent precipitation next time, make sure to store your lysis buffer in a room that is not too cold.
To increase the yield of a DNA/RNA extraction with a CleanNA kit, you can try pre-heating the elution buffer to 70 °C before elution.
Did you know disruption of cells, especially Gram-positive bacteria is crucial in order to obtain good DNA and/or RNA yields?
CleanNA’s Clean Pathogen DNA and RNA Kit comes with a specially designed disruptor plate containing a unique mix of beads for optimal cell disruption. Providing both high yield and high purity DNA and RNA from various clinical sample sources such as stool, serum, tissue or urine.
Please click here to request the SDS that you need.