DNA and RNA clean-up for next generation sequencing library construction
Since its introduction in 2005, Next Generation Sequencing (NGS) opened many doors in the fields of translational genomics and molecular diagnostics by massive parallel decoding of DNA or RNA fragments. To generate high quality NGS data, preparation of pure DNA or RNA of a specific length is one of the key process steps. We offer our CleanNGS for library cleanup and size selection to make this process simple and reliable.
Our special buffer formula ensures optimal size selection for NGS libaries and the high quality magnetic beads allow faster separations and better RNA/DNA recovery. CleanNGS is produced RNase free, which makes it an ideal solution for all downstream RNA or DNA NGS experiments.
CleanNGS is suited for cleanup in between library prepping steps, after library prepping and for size selection in next-generation sequencing or sanger sequencing. It is also a highly efficient cleanup method to use before other techniques such as PCR, cloning or CRISPR-Cas9 to improve the quality of your results. Our purification reagent provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s)..
Based on CleanNA’s proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.
For double sided size selection, we first add CleanNGS reagent with magnetic beads in a certain volume ratio. Separate the large DNA or RNA fragments from the solution with a magnetic plate and add more CleanNGS reagent to the supernatant to clean up the small DNA fragments and inhibitors. After two washing steps, the purified DNA or RNA is eluted.
Amplicons purified with CleanNGS system are ready to be used in the following applications:
Genomic DNA clean up
RNA clean up
Restriction enzyme clean up
Application note download: Automated size selection with CleanNGS, the cost-effective alternative to AMPure XP (INTEGRA Bioscience)
Our quality management system is certified to EN-ISO 13485 by Bureau Veritas