AVAILABLE KIT FORMATS
|Catalog No#||Description||Number of Reactions*|
|CNGS-0001||CleanNGS (1 mL)||55|
|CNGS-0050||CleanNGS (50 mL)||2,777|
|CNGS-0500||CleanNGS (500 mL)||27,777|
*Based upon a 10 µl PCR reaction volume
DNA and RNA clean-up for next generation sequencing library construction
The CleanNGS kit offers a highly efficient magnetic bead based clean-up system for the purification of both DNA and/or RNA for next-generation sequencing and sanger sequencing workflows. It can also be used for CRISPR-Cas9. CleanNGS provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).
Based on CleanNA’s proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications such. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.
Amplicons purified with CleanNGS system are ready to be used in the following applications:
- Next-Generation Sequencing
- Genomic DNA clean up
- RNA clean up
- Fragment Analysis
- Restriction enzyme clean up
Jestin George, Tim Kahlke, Raffaela M. Abbriano, Unnikrishnan Kuzhiumparambil, Peter J. Ralph and Michele Fabris.
Anca Liliana Cismaru, Livia Grimm, Deborah Rudin, Luisa Ibañez, Evangelia Liakoni, Nicolas Bonadies, Reinhold Kreutz, Pär Hallberg, Mia Wadelius, EuDAC Collaborators, Manuel Haschke, Carlo R. Largiadèr and Ursula Amstutz.