CleanNGS

AVAILABLE KIT FORMATS

Catalog No# Description Number of Reactions*
CNGS-0005 CleanNGS (5 mL) 277
CNGS-0050 CleanNGS (50 mL) 2,777
CNGS-0500 CleanNGS (500 mL) 27,777

*Based upon a 10 µl PCR reaction volume

CleanNGS

DNA and RNA clean-up for next generation sequencing library construction

The CleanNGS kit offers a highly efficient magnetic bead based clean-up system for the purification of both DNA and/or RNA for next-generation sequencing and sanger sequencing workflows. It can also be used for CRISPR-Cas9. CleanNGS provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s).

Based on CleanNA’s proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications such. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf) utilizing your current protocol as well as being used manually.

Amplicons purified with CleanNGS system are ready to be used in the following applications:

  • Next-Generation Sequencing
  • PCR
  • Genomic DNA clean up
  • RNA clean up
  • Fragment Analysis
  • Microarrays
  • Restriction enzyme clean up
  • Cloning

Learn more about our magnetic plates, such as the 96 Well Magnet Plates.

Features
  • Designed for both DNA and RNA purification
  • Ideal for (double-sided) size selection for Next-Generation Sequencing
  • Efficient removal of unincorporated dNTPs, primers, primer dimers and other contaminants
  • No centrifugation or filtration
Reviews
Amazing results!
“These beads are easy to use, and are extremely effective in our applications. The beads produce high quality results and also produce extremely reproducible work. My research would not be the same without these cost-effective and efficient beads. I also appreciate how friendly and the willingness to help that all of the sales team has at GC Biotech to help provide me with what I want.
Michelle Michelsen, University of Exeter
Easy to use!
“This product was used as part of my research project in molecular epidemiology and I was really happy with the results it produced. It made it an easy process to extract the DNA across all samples and ensured accurate sequencing data.”
Jonathan Abeles, London School of Hygiene and Tropical Medicine
Like-for-like replacement to the large name-brand equivalents.
“Delivers reproducible DNA size-selection for a variety of NGS applications and can be used in with-bead applications.”
Patrick van Eijk, School of Medicine